Principles and methods in the development of HPLC-DAD simultaneous determination of potent antioxidants resveratrol, vitamin E and coenzyme Q10 in capsules with normal phase separation columns

Abstract

The ultimate targets were development method with isocratic mode of separation yielding symmetrical peak shapes for the best sensitivity and accuracy, with shortest run time and best reproducibility. From analytical point of view important for liquid chromatography, the three analytes have quite distinct characteristics which contribute to successful method development. These features are their organic solvent and water solubility’s, molecular polarities and UV-absorption characteristics, like spectra and absorptivity’s. All these mentioned parameters were taken in account for solving complications appearing in development of rapid liquid chromatography method for the simultaneous determination of three antioxidant molecules.